. Die biologische Bulletin. Biologie; Zoologie; Biologie; Meeresbiologie. c > CJ> Q-Q-CO CO CO O< r • ^ I< r 200 r - 50 100 50 Kritische FENSTER FÜR Sr 72-96 hr L (2 EJ 3 24 L 62 67 72 80 96 120 0 ppm Sr 8 Sr 8 Sr/1 144 ich ich! F& £ Stunden von EIABLAGE Abbildung 4. Die alkalische Phosphatase Analyse. (A) Licht Schliffbild mit dunklen histochemische Färbung für alkalische Phosphatase Aktivität im. prevelar Lappen (PV) und Shell (sf) ofAplysia californica Embryo. 72 Uhr. von ASW + 8 ppm Sr. (B) wie in (einer für 72 Uhr Embryos von ASW) ohne Sr. (C) SEM-Aufnahme zeigt Wirkung von
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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. c_> CJ> Q- CO CO Q- CO O <r •^ _i <r 200 r- 50 100 50 CRITICAL WINDOW FOR Sr 72-96 hr L (2EJ3 24 L 62 67 72 80 96 120 0 ppm Sr 8 ppm Sr 8 ppm Sr/Be 1 144 I i! r &£ HOURS FROM OVIPOSITION Figure 4. Alkaline phosphatase analysis. (A) Light micrograph showing dark histochemical staining for alkaline phosphatase activity in the. prevelar lobes (pv), and shell field (sf) ofAplysia californica embryo. 72 h. from ASW + 8 ppm Sr. (B) as in (A) for 72 h embryo from ASW without Sr. (C) SEM micrograph showing effect of 0.09 ppm Be on shell formation of embryonic Aplysia californica. shells were malformed and crenulated (arrowheads), (scales = 10 ^m). (Graph) Specific alkaline phosphatase activity (normalized to total protein) of animals from the indicated treatments (mean ± S.D., n = 3). Only three time points were collected and analyzed for the 8 ppm Sr/0.09 ppm Be treatment. The largest increment in total alkaline phosphatase specific activity as measured biochemically was observed during the critical window in organisms from both the 0 and 8 ppm Sr treatments. 1 1 y = 156 168 192 nesium may substitute for strontium in vitro although the stringent specificity for the latter in vivo argues against this possibility. A defect in the organic matrix could severely limit or inhibit nucleation, but we were unable to demonstrate any morphological abnormalities in the organic shell nor a diminution of sulfated mucopolysaccharide synthesis in those embryos reared in the absence of strontium. The. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1
