. Die biologische Bulletin. Biologie; Zoologie; Biologie; Meeresbiologie. 622 MIROSLAV CESKA UND JAMES R. FISHER Ergebnisse in Tabelle IV beschrieben, kann mit der Annahme, dass die gewebehomogenaten effektiv Mischungen von Enzym und Inhibitor (s), das Enzym-Hemmer Interaktion reversibel ist, erklärt werden. Hinweise auf eine Reversibilität erreicht ist, der 4-Tag der Embryo als eine Quelle der arginase Aktivität und Herzen von 6 Wochen alten Hühnern zu hemmen. Ergebnisse in Abbildung 5 zeigen, dass dieses System die Eigenschaften, die laut Ackermann und Potter (1949) sind charakteristisch o Exponate

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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 622 MIROSLAV CESKA AND JAMES R. FISHER Results described in Table IV can be explained with the assumption that tissue homogenates are effectively mixtures of enzyme and inhibitor(s), provided the enzyme-inhibitor interaction is reversible. Evidence for reversibility has been obtained, using 4-day embryo as a source of arginase activity and heart from 6-week-old chickens to inhibit. Results presented in Figure 5 show that this system exhibits the properties which, according to Ackermann and Potter (1949), are characteristic of reversible inhibition. If tissue homogenates are effectively mixtures of enzyme and a reversible inhibitor (s), mixtures should exhibit activities equal to or less than the sum of the components but equal to or greater than the. cri e> MGS. EMBRYO/ML. REACTION MIXTURE FIGURE 5. Effect of heart from 6-week-old chickens (120 mg. per ml. reaction mixture) on the arginase activity of 4-day embryo at various concentrations. Activated with 5 XlO~* M Mn++. O embryo alone, X plus heart. activities of the least active components. Results in Table IV are consistent with these requirements. If the activity of a tissue homogenate is determined by the presence of a reversible inhibitor as well as enzyme, it is possible that activity would not be proportional to tissue concentration. Data in Figure 5 show that the argi- nase activity of 4-day embryos is proportional to tissue concentration over a 10-fold range. Such a proportionality has been shown by Straus and Goldstein (1943) when the ratio of inhibitor concentration to dissociation constant is greater than 100 (zone c). Our results can be explained on the basis of zone c inhibition. Assuming that homogenates are effectively mixtures of enzyme and inhibi- tor^), the data in Table IV require that different tissues have different inhibitor (s)-. Please note that these images are extracted from scanned page images that may have been dig

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