. Die biologische Bulletin. Biologie; Zoologie; Biologie; Meeresbiologie. 30 31 NIC jtmm. • 7 PC uc Abbildungen 27-31. Fm Holoihuriti knli. Erkennung von proliferierenden Zellen im regenerierenden C'uMcriun Tubuli. CL, Bindegewebsschicht; IE. interne Epithel; I., Lumen: M. mesolhehum; MC". mvocyte; P 2, Typ 2 pseiKlnpiulial Zelle. PC. periloneocyte; UC, unditTerenliatcil Zelle. Abbildung 27. Ich. ongiludinal Abschnitt ihnmgh ein st.ige 2 Regenerierende tuhule (BrdU/anti-BrdU Artisten zeigen beschriftet die Atomkerne in die drei Gewebeschichten (pfeilspitzen: bar=?() fimi. Abbildung 28. Detaillierte Ansicht der Wand ol ein s

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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 30 31 NIC jtmm. •7 PC uc Figures 27-31. Holoihuriti fm knli. Detection of proliferating cells in the regenerating C'uMcriun tubules. CL, connective tissue layer; IE. internal epithelium; I., lumen: M. mesolhehum; MC". mvocyte; P2, type 2 pseiKlnpiulial cell. PC. periloneocyte; UC, unditTerenliatcil cell. Figure 27. I .ongiludinal section ihnmgh a st.ige 2 regenerating tuhule (BrdU/anti-BrdU methodi showing labeled nuclei in the three tissue layers (arrowheads: bar = ?() fimi. Figure 28. Detailed view of the wall ol a stage 2 regenerating tuhule (Brdtl/anli-BrdU method) showing labeled nuclei in the mcsothclmm (single arrowhead) and in the inner epithelium (double arrowhead) (bar = 5 /urn). Figure 29. Section through the wall of a stage 3 regenerating tuhule (BrdU/anti-Brdf method) showing labeled nuclei in the mesothelium (peritoneocyte. single arrowhead: and unditterentiated cell, double arrowhead) and in the conncclixc tissue laser (type 2 pseudopodial cell, arrow) (bar = 8 ^tm). Figures 3(1 and 31. Consecutne scniilhm and ultialhin sections through a stage 3 I. rating tuhule. *HT incorporation in the tuhule tissues was icxealed In auloradiography on the semithin section (arrowheads. I ig 2°-: bar = 5 Jim) and the DNA-synthesi/ing cells wcie unambiguously identified by observation of the ultralhm section in I1-A1 il-'ig. 30: bar = 4 ^m). from the mesothelium. However, they had no direct e i deuce to corroborate their hypothesis. Our studs of regen- erating luhules clearly iiulicales ihc mesothelial origin of the myocytes which, during the regenerative process, first dif- ferentiate as myoepithelial cells and then migrate in the connective tissue layer. This is consistent with the hypoth- esis of Kicger and Lombard! (19X7) on the mesothelial origin ol nonepiihelial myocyles in echinoderms. This spec- ulative hypothesis was critici/ed hy Cavey and Wood (IWO), hut has since been strengthe

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• booksubjectzoology
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• buchsubjektbiologie